Casting a critical eye on plant stress research
For the benefit of learning
… by Abraham Blum
o ... and a letter to Plant Physiology – 2002
o The disturbing pot size effect in evaluating plant response to drought stress. (pots with water deficit raises the need to carefully consider the results)
DEFECTIVE PROTOCOLS IN STRESS RESEARCH
O Improper measurements of plant water status are still being used (in Physiologia Plantarum)
O Improper measurements of plant water status are still being used (#2 ) (in Euphytica)
O Irrelevant stress protocols used and reported in genomic research (in Plant Molecular Biology)
O Irrelevant stress protocols used and reported in genomic research-II (in Planta)
O Physiological and developmental problems encountered by in vitro cultured plants (in Plant Growth Regulation)
O In a genomics study drought stress was applied to leaf discs dried in air for 2 hours (in Theoretical and Applied Genetics)
O … And still unrealistic drought stress treatment in a genomics study – seedlings dried for 0.5 to 2h (in Theoretical and Applied Genetics)
O Irrelevant drought stress treatments and interpretations in plant physiology experiments – still in view (in Plant Growth Regulation)
O Gene expression under drought stress is assessed by pulling seedlings out of nutrient solution and desiccating them in air. (in Journal of Experimental Botany)
O A new inventive “drought stress” protocol (in Molecular Breeding)
O Proline and drought resistance (in Frontiers of Plant Science)
CONTRARIAN DATA AND VIEWS
O “Salt stress or salt shock: which genes are we studying?” – Issues with salinity gene-expression research.
O ROS are good. A view (by Ron Mittler, 2017).
O Regulating Tradeoffs to Improve Rice Production – Certain genes may have both positive and negative effects on crop performance
I SAY, WHAT?
o Extensive media reports (Oct.2017) about a salt resistant rice growing in “sea water” (China) -- (one example - read carefully)