Again- an improper drought stress protocol for testing
transgenic plants
In their report in Planta (2009) 229:605–615 (Free access-- http://www.ricedata.cn/reference/pdfiles/0082v229i3p605.pdf ) Guojun Lu,Chenxi Gao, Xingnan Zheng
and Bin Han test their rice plants for drought resistance by subjecting them to
9.5 hours in PEG 20%
solution (see Fig.6). It can be suspected that the protocol of 9.5 hours in PEG has been adopted from some practice used with
cell cultures. PEG might be
appropriate or not for stressing rice plants (see our Methods page), but a 9.5
hour stress period is un-natural and irrelevant. It does not allow plants to express
their full capacity for adaptation. At least a period of one week until
stress symptoms appear is more reasonable.